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fap af647  (R&D Systems)


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    Structured Review

    R&D Systems fap af647
    Fap Af647, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+fap/Mouse+Fibroblast+Activation+Protein+alpha%2FFAP+Alexa+Fluor%C2%AE+647-conjugated+Antibody/pm41714703-270-55-57
    Average 94 stars, based on 1 article reviews
    fap af647 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI‐1‐TGF‐ β ‐myoCAF‐Dependent Mechanism
    Article Snippet: Fresh tumor tissues from Bmal1 +/+ and Bmal1 −/− mice were cut into small pieces and incubated in a combination with 0.15% type I collagenase (Cat. C0130‐500MG, Sigma‐Aldrich) and 0.15% type II collagenases (Cat. C6885‐1G, Sigma‐Aldrich) in PBS at 37 °C for 1 h. Digested tissues were filtered with a 100 μm cell strainer and followed by a 70 μm cell strainer. .. For FAP + CAFs sorting, after centrifugation, single‐cell suspensions from each sample were stained on ice for 15 min with a rat anti‐mouse FAP (1:50, Cat. MAB9727, R&D system) antibody, followed by an Alexa Fluor 647‐conjugated donkey anti‐Rat secondary antibody (Cat. A48272, Thermo Fisher Scientific). ..

    Single Cell:

    Article Title: Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI‐1‐TGF‐ β ‐myoCAF‐Dependent Mechanism
    Article Snippet: Fresh tumor tissues from Bmal1 +/+ and Bmal1 −/− mice were cut into small pieces and incubated in a combination with 0.15% type I collagenase (Cat. C0130‐500MG, Sigma‐Aldrich) and 0.15% type II collagenases (Cat. C6885‐1G, Sigma‐Aldrich) in PBS at 37 °C for 1 h. Digested tissues were filtered with a 100 μm cell strainer and followed by a 70 μm cell strainer. .. For FAP + CAFs sorting, after centrifugation, single‐cell suspensions from each sample were stained on ice for 15 min with a rat anti‐mouse FAP (1:50, Cat. MAB9727, R&D system) antibody, followed by an Alexa Fluor 647‐conjugated donkey anti‐Rat secondary antibody (Cat. A48272, Thermo Fisher Scientific). ..

    Staining:

    Article Title: Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI‐1‐TGF‐ β ‐myoCAF‐Dependent Mechanism
    Article Snippet: Fresh tumor tissues from Bmal1 +/+ and Bmal1 −/− mice were cut into small pieces and incubated in a combination with 0.15% type I collagenase (Cat. C0130‐500MG, Sigma‐Aldrich) and 0.15% type II collagenases (Cat. C6885‐1G, Sigma‐Aldrich) in PBS at 37 °C for 1 h. Digested tissues were filtered with a 100 μm cell strainer and followed by a 70 μm cell strainer. .. For FAP + CAFs sorting, after centrifugation, single‐cell suspensions from each sample were stained on ice for 15 min with a rat anti‐mouse FAP (1:50, Cat. MAB9727, R&D system) antibody, followed by an Alexa Fluor 647‐conjugated donkey anti‐Rat secondary antibody (Cat. A48272, Thermo Fisher Scientific). ..

    Article Title: Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI‐1‐TGF‐ β ‐myoCAF‐Dependent Mechanism
    Article Snippet: .. After boiling with antigen unmasking solution (H3300, Vector) for 20 min and subsequently blocked with 4% serum, tissue slides were stained at 4 °C overnight with a rat anti‐mouse FAP (1:200, Cat. MAB9727, R&D system) antibody, a mouse anti‐mouse α ‐SMA (1:200, Cat. MA5‐11547, Thermo Fisher Scientific) antibody, a goat anti CD31 (1:400, Cat. AF3628, R&D system) antibody, a rat anti ENDOMUCIN (1:400, Cat. 14 585 185, eBioscience) antibody, a rabbit anti DESMIN (1:200, Cat. 0 4585, Millipore) antibody, a goat anti PDGFR α (1:200, Cat. AF1062, R&D system) antibody, a rabbit anti PDGFR β (1:200, Cat. 13449‐1‐AP, Proteintech) antibody, a rabbit CAIX (1:200, Cat. NB100‐417, NOVUS) antibody, a rat anti Ki67 (1:400, Cat. 14‐5698‐82, eBioscience) antibody, a rabbit anti F4/80 (1:200, D2S9R, Cell Signaling TECHNOLOGY) antibody, a mouse anti CD80 (1:200, Cat. NBP2‐25255SS, NOVUS BIOLOGICALS) antibody, and a mouse anti CD206 (1:200, Cat. 60143‐1‐Ig, Proteintech) antibody, followed by staining for 1 h at room temperature with species‐matched secondary antibodies. .. Apoptotic staining was performed according to the manufacturer's protocol (Cat. 11 684 795 910, Roche).



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